Tapas Chakraborty
PhD Mumbai, India National Institute for Basic Biology (NIBB, Japan)
Title: Comparative transcriptomics and Edwardsiellosis detection in fish: a study using medaka and Red Sea bream
Biography
Biography: Tapas Chakraborty
Abstract
Fish has been recognized as the most promising and sustainable source of future food security. However, disease outbreak and slow and improper diagnosis often hinders the profitable blue-crop. Interestingly, global diseases like edwardsieallosis affects many commercially important fish. In order to find common remedial measures to curb Edwardsiealla infection in fish, a general idea of how the infection affects an individual, especially during early stages is highly essential. Using several in vivo experiments, we have previously shown that Hepcidin1, a gene critically involved in the starvation associated disease management, is a critical biomarker for E. tarda infection in Pagrus major. Owing to E. tarda’s vast infection terrain and enormous host susceptibility, in the present investigation, we aimed to determine the early molecular pathogenesis of E. tarda in a model (medaka) and commercially important (Red sea bream) fish. To achieve our goal, we infected medaka and red sea bream with E. tarda (@ 109CFU/ml water) and performed comparative transcriptome analysis using 6 and 24 hours post infected liver samples.