Khaled M. Selim
Kuwait Institute for scientific research, Kuwait
Title: Isolation and identification of the potential probiotics from the gut of the African catfish, Clarias gariepinus
Biography
Biography: Khaled M. Selim
Abstract
The aim of this study is to isolate beneficent probiotic bacteria from the gut of the African catfish, Clarias gariepinus. This study could be the first specific study to isolate and characterize indigenous potential probiotic strains from the gut of the African catfish. Eighty-nine isolates from Clarias gariepinus gut were screened for in-vitro antibacterial activities against five fish pathogens, strains Aeromonas sobria (A. sobria), Aeromonas hydrophila (A. hydrophila), Pseudomonas aeruginosa (P. aeruginosa), Pseudomonas putida (P. putida) and Staphylococcus aureus (S. aureus). Seven isolates had antibacterial activities with at least one pathogen, and safe after injection intraperitoneally. Confirmatory tests showed that such isolates can survive in the presence of high bile concentrations (10%) and at highly acidic pH. Three strains are sensitive to selected antibiotics. Four and three out of seven strains had amylase and protease activities, respectively. Based on morphological, biochemical tests and 16S rRNA gene analysis, the isolated strains were identified as follows: B. subtilis; Bacillus amyloliquefaciens, Bacillus cereus, Citrobacter freundii (2 isolates), Leuconostoc sp. and Edwardsiella sp.