Farzaneh Farokhi
Islamic Azad University of Sari, Iran
Title: The single-cell gel electrophoresis assay to determine apoptosis induced by blood cells in Rutilus rutilus caspicus
Biography
Biography: Farzaneh Farokhi
Abstract
The present study aimed to determine the biomarkers of malathion in Rutilus rutilus caspicus by studying genotoxicity and ecophysiologic reactions. To achieve this goal, the lethal concentration (Lc50) of malathion in Rutilus rutilus caspicus was examined. The effect of different 0.1%, 0.05% and 0.01% ppm concentrations on DNA of red blood cells of this species was observed, following the sampling which was done in the 3rd, 13th and 23th days of malathion exposure and 30 days after the exposure. Although the study did not show any significant deviation regarding the number of apoptotic cells in some types of exposed specimens to malathion (P>0.05), a significant difference was observed between the control group and the treatments in all days of sampling (P<0.05). It has also been indicated that Rutilus rutilus caspicus were sensitive to malathion and the induced apoptosis was dose dependent and increased by extending the periods of exposure.