10^th International conference on Fisheries & Aquaculture September 27-28, 2019 Park Inn by Radisson Hotel, Toronto, Ontario, Canada

Biography:

Tapas Chakraborty has completed his PhD at the age of 28 years from Central Institute of Fisheries Education (Mumbai, India) and National Institute for Basic Biology (NIBB, Japan) in 2010. He has done his postdoctoral studies from NIBB and Ehime University (Japan) and is presently working as an Assistant Professor at Ehime University, Japan. He has published more than 22 papers in reputed journals, obtained 2 patents and has been serving as an editorial board member of repute in several esteemed journals. He has received more than 10 awards and scholarships, and has worked as lead/co-lead in 10 projects

Abstract:

Fish has been recognized as the most promising and sustainable source of future food security. However, disease outbreak and slow and improper diagnosis often hinders the profitable blue-crop. Interestingly, global diseases like edwardsieallosis affects many commercially important fish. In order to find common remedial measures to curb Edwardsiealla infection in fish, a general idea of how the infection affects an individual, especially during early stages is highly essential. Using several in vivo experiments, we have previously shown that Hepcidin1, a gene critically involved in the starvation associated disease management, is a critical biomarker for E. tarda infection in Pagrus major. Owing to E. tarda’s vast infection terrain and enormous host susceptibility, in the present investigation, we aimed to determine the early molecular pathogenesis of E. tarda in a model (medaka) and commercially important (Red sea bream) fish. To achieve our goal, we infected medaka and red sea bream with E. tarda (@ 10⁹CFU/ml water) and performed comparative transcriptome analysis using 6 and 24 hours post infected liver samples.

Biography:

I am  Shimaa Salem a visiting research scholar at school of Fisheries, Aquaculture and Aquatic sciences, Auburn University,AL,USA.I am working in fish nutrition projects, especially protein and amino acids nutrition in channelcatfish and tilapia.Also, I have worked in trace mineral and lipid nutrition researches. I am a coauther in two published papers in reputed journals

Abstract:

Eight weeks growth study was performed to reduce the intact protein (IP) content in channel catfish Ictalurus punctatus diet with balancing the amino acids profile by adding of indispinsable amino acids (IAA) at graded levels.Also, assessment the contribution of  dispinsable amino acids (DAA; glutamic acid & glycine) supplementation to the low intact protein diet. Nine  isolipidic (8% lipid) experimental diets were formulated  as the following;  (30%IP basal  , 24%IP/ IAA were added  to restore IAA profile the same as basal diet+DAA), (24%IP/IAA were added at different levels 100, 120 &140% NRC 2011 ), (24, 27 & 30%IP/ IAA were added at 120% NRC), (24%IP/ IAA were added at 100%NRC with/without DAA, 24%IP/IAA  were added at 120% NRC with/without DAA). Juvenile catfish fish (average initial weight 23.68 g) were randomly allocated into 9 groups,

Biography:

Abstract:

Biography:

Dr. Mozhgan Sepehri is an Assistant Professor of plant-microbe interactions at Shiraz University, Iran. Dr. Sepehri’s research program provides novel insights into host adaptation to environmental stresses induced by plant microbial communities. She incorporates biotechnological approaches such as proteomics and metabolomics to decipher the molecular mechanisms of induced abiotic stresses in plants by beneficial microorganisms. She seeks a fuller understanding of the phytobiome as a natural supply of bacteria, fungi and other microorganisms with the individual or collective capacity to promote plant health. 

Abstract:

Abiotic stresses are the the most important challenges facing agriculture, due to preventing plants from realizing their full genetic potential for growth and reproduction. Hence, improving plant growth and productivity under stress conditions has gained high agricultural priority worldwide as the environmental stresses persistently limit choice of crops and agricultural production in many areas of the world. Over the past decades, advanced molecular techniques have been used to develope new crop varieties with enhanced stress tolerance. Benefits aside, molecular breeding as an effective trait in improvement of several crops comes with many potential drawbacks which significantly limit its potential outcomes. The application of some plant-associated microorganisms, including endophytic fungi opened up new possibilities for improving stress adaptation of the host plants growing under environmental stresses. In recent years, several studies have been carried out to decipher the molecular mechanisms underlying endophytes-mediated stress tolerance in agriculturally important plants. In the postgenomic era, omics-based approaches including genomics, proteomics, transcriptomics, and metabolomics have been widely used as powerful techniques in the field of abiotic stress tolerance in crops; since they allow rapid identification of specific genes, proteins and metabolites that contribute to better plant performance under stress conditions. Also, plant endogenous small RNAs, including microRNAs (miRNAs) and small interfering RNA (siRNAs) play an important role in regulating plant defense machinery against environmental stresses. New information about the role of miRNAs during plant-microbe interaction led to exploiting the novel miRNAs and their targeted genes to generate stress-tolerant crops in future.

Biography:

Sipra Mohapatra has completed her PhD from Central Institute of Fisheries Education (Mumbai, India) in 2010. She has done her postdoctoral studies from Southwest University (China), and Ehime University (Japan) and is at present working as an Assistant Professor at Ehime University, Japan. She has published 19 peer-reviewed articles and written 2 book chapters and is a reviewer in 4 reputed journals. She is the recipient of more than 5 awards and scholarships and has worked as lead/co-lead in over 10 projects.

Abstract:

Autophagy is a cellular process that delivers cytoplasmic material to the lysosome for recycling. It is stimulated above the basal or resting rate when nutrients are scarce, cells are under stress, or damaged organelles need to be degraded. High throughput transcriptional profiling suggested that autophagic involvement in early disease resistance and stress management differed between male and female fish. Since, sex steroid and their responsive receptors, especially estrogen and estrogen receptors (ERs), abundances are sexually dimorphic, we deduced that sex-biased autophagy may be regulated by ERs. To prove that, we used ER and ER2 knockout (KO) medaka and analyzed the alterations in the autophagic genes and protein expression in the liver and gonad. We found significantly increased mTOR expression in ER-KO, but not in ER2-KO female fish. This suggested differential involvement of ERs in autophagic regulation, which was further confirmed by ULK and Beclin transcription, and mitochondrial population. Interestingly, the LC3 (the last major autophagy factor) contents/cell and LC3 positive cells were increased significantly in ER-KO fish. In-depth analysis showed that LC3 nuclear-cytoplasmic transports were partially (ER2-KO) or completely (ER-KO) compromised due to SIRT/DOR protein regulation in the nucleus. We also found that autophagy is not only instrumental in germ cell degeneration but also important for oocyte and sperm formation/development in the ER-KO fish. Cumulatively our data highlights the sexbiased autophagy and ER association, stress-influenced apoptosis/autophagy cell fate decision, and the immense significance of autophagy in fish liver and gonad physiology.

Biography:

Sivan Klas has completed his PhD in Environmental Engineering from the Technion IIT (2010) and postdoctoral studies in the Department of Chemical Engineering at the University of Toronto (2012). He has published more than 10 papers in reputed journals. He is currently a Senior Lecturer at the Department of Biotechnology Engineering, ORT Braude College of Engineering and a Management Committee member in a European Cooperation in Science and Technology (COST) Action.

Abstract:

Construction of groundwater desalination plants in arid and semi-arid areas is increasing. Already in Israel, millions of cubic meters of brine are produced each year and more are expected shortly. This brine may be used as a new water source for aquaculture, and draws high interest by Israeli inland fish farmers, due to its low cost, constant temperature and pathogen-free characteristics, as well as the opportunity of growing marine species inland. The experience in using groundwater desalination brine for aquaculture is, however, very limited. In unpublished experience, scale formed on essential organs and led to fish mortality. In the current study, sea-bass survival in brine that was pretreated by aeration to reduce calcium concentrations (“softening”) was investigated. Kinetics of the softening process were also studied to assess design criteria for commercial application. Results suggested that under very low water exchange rates, fish can survive even in untreated brine. Over 70% of calcium can be removed in the process, at a hydraulic retention time of several hours. CaCO3 was found to be the dominant precipitant, at up to 1.5kg m-3. The aeration rate was found to have a much stronger effect on kinetics, compared with seed concentration. It was concluded that fish rearing in desalination brine may be feasible and economic, although care must be taken about the exchange rate and biofiltration. A pilot system is planned to operate during 2019 near Kfar Massarik desalination plant in northern Israel.

Biography:

Nadiyeh Rouhi doing her PhD at Tarbiyat Modares University. She also is fisheries expert at Chabahar Free zone organization and consulting fisheries investors. she is an active, intelligent and goal-oriented individual; she revealed diligence and accuracy along with satisfaction by her work. The aforementioned characteristics can be well manifested on the grade of their final thesis. In addition to academic and research abilities, she has excellent communication skills so she can easily work and cooperate with her coworkers. She has been independently conducting her main research in addition to collaboration with other research fellows on the other related projects.

Abstract:

In this study, fish oil was extracted from precooked and non-precooked longtail tuna (Thunnus tonggol) heads using hot air cooking at 80ËšC for 30min and extraction yield was measured. To determine fish oil quality, peroxide value (PV), thiobarbituric acid (TBA), free fatty acids FFA), conjugated dienes (CD), and fatty acid composition was measured in the treatments. The results showed the highest yield in non-precooked heads. Oils obtained from the non-precooked heads showed lower PV, TBA, FFA, and CD compared to the precooked heads. However, a higher amount of DHA polyunsaturated fatty acid (27.50%) was found in oils obtained from precooked heads in comparison with non-precooked heads (18.52%). Crude oil samples were obtained from both the precooked and non-precooked heads contained high levels of palmitic acid (C16:0), oleic acid (C18:1), DHA (C22:6), meristic acid (C14:0), palmitoleic acid (C16:1), stearic acid (C18:0), behenic acid (C22:0). In conclusion, oils obtained from the non-precooked samples had higher yields and better quality while the DHA was higher in the oils obtained from precooked samples.

Biography:

Tapas Chakraborty has completed his PhD at the age of 28years from Central Institute of Fisheries Education (Mumbai, India) and National Institute for Basic Biology (NIBB, Japan) in 2010. He has done his postdoctoral studies from NIBB and Ehime University (Japan) and is presently working as an Assistant Professor at Ehime University, Japan. He has published more than 22 papers in reputed journals, obtained 2 patents and has been serving as an editorial board member of repute in several esteemed journals. He has received more than 10 awards and scholarships and has worked as lead/ co-lead in 10 projects. 

Abstract:

Fish has been recognized as the most promising and sustainable source of future food security. However, disease outbreak and slow and improper diagnosis often hinder the profitable blue-crop. Interestingly, global diseases like Edwardsiella affects much commercially important fish. To find common remedial measures to curb Edwardsiella infection in fish, a general idea of how the infection affects an individual, especially during early stages is highly essential. Using several in vivo experiments, we have previously shown that Hepcidin1, a gene critically involved in the starvation associated disease management, is a critical biomarker for E. tarda infection in Pagrus major. Owing to E. tarda’s vast infection terrain and enormous host susceptibility, in the present investigation, we aimed to determine the early molecular pathogenesis of E. tarda in a model (medaka) and commercially important (Red sea bream) fish. To achieve our goal, we infected medaka and red sea bream with E. tarda (@109CFU/ml water) and performed comparative transcriptome analysis using 6 and 24hours post infected liver samples. We found that infection altered the complement system gene expression, vitellogenin production, translation initiation, energy metabolism, cell death, iron homeostasis, ion balance pathways. Further real-time PCR analysis confirmed that several genes especially Hepcidin1, Ubiquitin1 (Autophagy regulator), HSP70 (stress management), SDF2 (cell maintenance), SLC41a (Ion balance) and EIF3d (translation initiation) were altered in a time-dependent manner. Although more thorough investigations are pertinent, our present data suggest the possibility of a general E. tarda molecular pathophysiology in fish